Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine
نویسندگان
چکیده
A novel TaqMan 5-plex real-time PCR was developed for the identification of Brachyspira species in swine. B. hyodysenteriae, pilosicoli, and suanatina are identified using three hydrolysis probes targeting cpn60, while hampsonii is recognized by another nox specific probe. This highly sensitive assay easy to implement routine veterinary diagnostic laboratories enables rapid differentiation between main four pathogenic pigs, a single-tube approach.
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ژورنال
عنوان ژورنال: MicrobiologyOpen
سال: 2021
ISSN: ['2045-8827']
DOI: https://doi.org/10.1002/mbo3.1169